R. Paul Nobrega
Biophysical Scientist
Automation Consultant
Scientific Programmer
Education
PhD in Biophysics
University of Massachusetts
Medical School
2008-2015
BS in Biotechnology
University of Massachusetts
at Lowell
2004 - 2008
Career profile
PhD with 5 years of industry experience, over 15 years of laboratory experience, and 5 years of professional programming and laboratory automation experience.
Proven track record for novel research, leading multidisciplinary collaborative projects, and synthesizing large datasets into succinct conclusions.
Experience
Senior Scientist
2019 - Present
Institute for Protein Innovation, Boston MA
- Manage development of LIMS system
- Automate data acquisition and analysis, lab-wide (python, c#)
- Build custom microservices for custom functions/user interfaces
- Apply data science techniques to interrogate biological data
- Contribute to protein engineering projects
- Mass spectrometery of biomolecules
Scientist I / Scientist II
2015 - 2019
Adimab, Lebanon NH
- Conduct and evaluate accelerated stability assays on drug-candidate mAb molecules via MS/MS
- Automate data acquisition and analysis (python, c#)
- Use BLI for measuring binding kinetics and epitope mapping experiments
- Lead exploratory protein engineering projects
Automation Consultant, Co-Founder
2015 - Present
Boston AutoLytics, LLC
- Evaluate, prescribe, and develop automated solutions for data acquisition and analysis
- Projects are biopharma focused with clients including: Adimab, Avitide, and Eli Lilly
PERSONAL PROJECTS
Check out these on-going software projects:
PyVuka - A flexible and general purpose global fitting software for analyzing scientific data, written in python
ForteBioPKG - A python package for the automated analysis of high-throughput ForteBio BLI data. Automation includes kinetic and
epitope mapping analyses
SKILLS & PROFICIENCY
Data Analysis
Mass Spectrometry
Moelcular Kinetics
Chromatographic and Light Scattering/Absorption Techniques
Protein Engineering
Web Design & App production (php, html, javascript, jquery, etc)
Computer programming (Python & C#)
Specific Laboratory Techniques
NMR, Peptide Mapping, LCMS, MALDI, SPR, BLI, DSF, Micro-fluidic mixing, SAXS, CD, FL, PCR, HIC, CEX, SEC, Binding Kinetics, Folding Kinetics, MD, Intein Chemistry, Protein Engineering, Protein Purification, FRET, SDS-PAGE,
DLS
PRofessional PUBLICATIONS
Most of my professional publications are indexed on PubMed [Here]. Direct download links:
[13] Deamidation and isomerization liability analysis of 131 clinical-stage antibodies (2019)
[6] Modulation of frustration in folding by sequence permutation (2014)
[5] Microsecond barrier-limited chain collapse observed by time-resolved FRET and SAXS (2014)
[4] Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam (2013)
[2] Complementary techniques enhance the quality and scope of information obtained from SAXS (2013)